EAJAIC
Eurasian Journal of Anesthesiology and Intensive Care

The Eurasian Journal of Anesthesiology and Intensive Care (EAJAIC) is an independent-unbiased, peer-reviewed, "double-blind", and open-access journal of current national and international issues and reviews for original clinical and experimental research, interesting case reports, differential diagnoses, editorial opinions, letters to the editor, and educational papers in anesthesiology, algology, and intensive care medicine.

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Original Article
Examining the effects of lidocaine and dexmedetomidine on compound action potential in rat sciatic nerve
Abstract
Aims: Recent interest in Peripheral Regional Blocks (PRB) has increased for surgical and nonsurgical implementations. Lidocaine is frequently preferred in PRBs because of its wide safety margin. The short duration of action limits the use of Lidocaine. Dexmedetomidine is a new-generation adjuvant agent. According to our hypothesis, Dexmedetomidine will prolong the duration of the action of Lidocaine.
Methods: A total of 48 rat sciatic nerves were isolated and electrophysiological recordings were taken from 28. They were then randomly divided into 4 groups as Group Control (C), Group L given 1 mM Lidocaine, Group D given 5 microgram/L Dexmedetomidine, and Group LD given 1 mM Lidocaine and 5 microgram/L Dexmedetomidine. Compound Action Potentials (CAP) were recorded after the stimulation of the nerves. Maximum Depolarization Value (MD, mV), time to Maximum Depolarization (MDT), Area Under the CAP curve (Area, mV.ms), and conduction velocities were calculated separately for Fast-Conducting Fibers (CVF, m/s) and Medium-Slow-Conducting Fibers (CVM, m/s).
Results: In the comparisons of the Areas Under the Curve, significant differences were detected for Group C and Group LD (P=0.001). In the comparisons of CVF values, significant differences were detected for Group C with Group L and Group LD (p=0.007, p=0.000). In the comparisons of Group L and Group LD (p=0.002). In the comparisons of CVM values, significant differences were detected for Group C with Group L and Group LD (p=0.000, p=0.000, respectively). Significant differences were detected between Group L, and Group LD (p=0.009).
Conclusion: Results were found showing that perineural Dexmedetomidine when added to Lidocaine as an adjuvant agent, increased the number of fibers that developed block and decreased conduction velocities. These results might indicate that Dexmedetomidine may increase the duration of the block.

Keywords : Rat sciatic nerves, compound action potentials, lidocaine, dexmedetomidine

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Article available in :
Volume 2, Issue 1, 2025
Page : 18-23
https://doi.org/10.51271/EAJAIC-0024
Article Information
Received : 22 Kas 2024
Accepted : 27 Ara 2024
Published : 03 Şub 2025
Corresponding Author :

Hatice Toprak: Department of Anesthesiology and Reanimation, Faculty of Medicine, Karamanoğlu Mehmetbey University, Karaman, Turkiye

[email protected]
Citation : Toprak H, Öztin Öğün C. Examining the effects of lidocaine and dexmedetomidine on compound action potential in rat sciatic nerve. Eur J Anesthesiol Intens Care. 2025;2(1):18-23.

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